NASC Stock Detail Page
NASC ID: N849493
Name: WiscDsLox241F03
ABRC stock number: CS849493
Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line. Associated Polymorphisms: Wiscseq_DsLox241F03.0 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G63060.1 (chr 3 pos 23301546 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG796277. Wiscseq_DsLox241F03.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G10910.1 (chr 1 pos 3641316 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank ID: KG796275. Wiscseq_DsLox241F03.2 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT1G12910.1 (chr 1 pos 4395045 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG796276. Wiscseq_DsLox241F03.3 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT5G54920.1 (chr 5 pos 22301943 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG796278. Wiscseq_DsLox241F03.4 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G56520.1-UTR3 and AT5G56530.2-P355 (chr 5 pos 22888614 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG796279.
Donation Date: 2004-12-01
Donated by: | University of Wisconsin, Madison Patrick Krysan University of Wisconsin, Madison Richard Amasino University of Wisconsin, Madison Michael Sussman |
Donor Number: WiscDsLox241F03
Part of : Wisconsin DsLox T-DNA lines
Stock type: individual line
Material type: seed
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: T-DNA Insertion
Background: Col-2
Transformation method: Agrobacterium
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
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